Zona-free hamster ovum penetration by human spermatozoa: influence of various sperm factors.
نویسندگان
چکیده
The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemination. The use of control samples was necessary because of wide intraindividual and interindividual variations in the results. Unless the result has been confirmed several times, it is impossible to conclude, from a negative test, that a human spermatozoon cannot penetrate a hamster oocyte.
منابع مشابه
Ejaculate-hormonal traits in the leopard cat (Felis bengalensis) and sperm function as measured by in vitro penetration of zona-free hamster ova and zona-intact domestic cat oocytes.
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In this study we set out to characterize gp20, a 20 kDa glycoprotein of the human sperm surface, first identified by us by radiolabelling the sialic acid residues of the sperm surface [R. Focarelli et al. (1995), Mol. Hum. Reprod., 2, 2755-2759]. The protein was partially purified from pooled sperm samples of several healthy donors and used to raise a specific antiserum to study its localizatio...
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ورودعنوان ژورنال:
- Reproduction, nutrition, developpement
دوره 25 3 شماره
صفحات -
تاریخ انتشار 1985